A new method for detection of pandemic influenza virus using High Resolution Melting analysis of the neuraminidase gene

David Varillas; Jesus F Bermejo-Martin; Raquel Almansa; Silvia Rojo; Begoña Nogueira; Jose María Eiros; Lucia Rico; Verónica Iglesias; Raul Ortiz de Lejarazu

doi:10.1016/j.jviromet.2010.10.003

A new method for detection of pandemic influenza virus using High Resolution Melting analysis of the neuraminidase gene

J Virol Methods. 2011 Jan;171(1):284-6. doi: 10.1016/j.jviromet.2010.10.003. Epub 2010 Oct 15.

Authors

David Varillas¹, Jesus F Bermejo-Martin, Raquel Almansa, Silvia Rojo, Begoña Nogueira, Jose María Eiros, Lucia Rico, Verónica Iglesias, Raul Ortiz de Lejarazu

Affiliation

¹ National Influenza Centre, Valladolid, Spain. [email protected]

PMID: 20951745
DOI: 10.1016/j.jviromet.2010.10.003

Abstract

Diagnostic methods based upon exclusive detection of haemagglutinin do not detect sequence variation in other gene segments of the Influenza A virus. A complementary approach is described based upon high-resolution melting curve analysis of the neuraminidase gene, an approach with the potential ability to detect small changes in the neuraminidase sequence without the need for specific probes.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Agar Gel / methods
Humans
Influenza A Virus, H1N1 Subtype / genetics
Influenza A Virus, H1N1 Subtype / isolation & purification*
Influenza, Human / diagnosis*
Neuraminidase / genetics*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Transition Temperature*
Viral Proteins / genetics*
Virology / methods*

Substances

Viral Proteins
NA protein, influenza A virus
Neuraminidase