Role of GPR30 in endometrial pathology after tamoxifen for breast cancer

Am J Obstet Gynecol. 2010 Dec;203(6):595.e9-16. doi: 10.1016/j.ajog.2010.07.034. Epub 2010 Oct 20.

Abstract

Objective: This study was undertaken to evaluate the potential role of G-protein-coupled estrogen receptor in endometrial pathology associated with tamoxifen treatment of breast cancer patients.

Study design: We investigated whether G-protein-coupled estrogen receptor plays a role in mediating proliferating effect of tamoxifen in endometrial carcinoma cells. These results were compared with the G-protein-coupled estrogen receptor expression pattern in endometrial tissue from a cohort of 95 breast cancer patients, who received tamoxifen or another adjuvant therapy.

Results: In vitro tamoxifen significantly stimulated the mitogen-activated protein kinase phosphorylation and cell proliferation of endometrial cell lines via G-protein-coupled estrogen receptor. In vivo, there was a significant correlation between G-protein-coupled estrogen receptor expression and the tamoxifen-induced endometrial pathology (P = .006). Moreover, G-protein-coupled estrogen receptor positivity was predictive of an earlier development of symptoms, such as bleeding or suspect endometrial thickness, induced by tamoxifen therapy (P = .019).

Conclusion: G-protein-coupled estrogen receptor plays an important role in tamoxifen-induced endometrial abnormalities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / pathology
  • Cell Proliferation / drug effects
  • Endometrial Neoplasms / chemically induced*
  • Endometrial Neoplasms / pathology*
  • Female
  • Humans
  • Immunohistochemistry
  • In Vitro Techniques
  • Receptors, G-Protein-Coupled / metabolism*
  • Reference Values
  • Sensitivity and Specificity
  • Tamoxifen / adverse effects*
  • Tamoxifen / pharmacology
  • Tamoxifen / therapeutic use
  • Tumor Cells, Cultured / drug effects

Substances

  • Receptors, G-Protein-Coupled
  • Tamoxifen