Serological evidence of pig-to-human influenza virus transmission on Thai swine farms

Pravina Kitikoon; Donruethai Sreta; Ranida Tuanudom; Alongkorn Amonsin; Sanipa Suradhat; Kanisak Oraveerakul; Yong Poovorawan; Roongroje Thanawongnuwech

doi:10.1016/j.vetmic.2010.09.019

Serological evidence of pig-to-human influenza virus transmission on Thai swine farms

Vet Microbiol. 2011 Mar 24;148(2-4):413-8. doi: 10.1016/j.vetmic.2010.09.019. Epub 2010 Sep 29.

Authors

Pravina Kitikoon¹, Donruethai Sreta, Ranida Tuanudom, Alongkorn Amonsin, Sanipa Suradhat, Kanisak Oraveerakul, Yong Poovorawan, Roongroje Thanawongnuwech

Affiliation

¹ Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd., Bangkok 10330, Thailand.

PMID: 20965670
DOI: 10.1016/j.vetmic.2010.09.019

Abstract

We investigated influenza interspecies transmission in two commercial swine farms in Thailand. Sera from swine-exposed workers (n=78), age-matched non-swine-exposed healthy people (n=60) and swine populations in both farms (n=85) were studied. Hemagglutination-inhibition (HI) assay was performed on Thai swine H1 viruses (swH1N1 and swH1N2) isolated from both farms. Thai human H1N1 (huH1N1) and pandemic H1N1 2009 (pH1N1) were also used as test antigens. The hemagglutinin (HA) 1 genes of swH1N1 and swH1N2 viruses were sequenced and shown to be genetically distinct from the Thai huH1N1 and pH1N1 viruses. Evidence of pig-to-human influenza virus transmission was found in farm workers with increased odds of elevated antibody titers to both swH1N1 (OR 42.63, 95% CI, 14.65-124) and swH1N2 (OR 58, 95% CI, 13.12-256.3) viruses. No evidence of human-to-pig influenza virus transmission was detected in this study.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Animals
Antibodies, Viral / blood
Female
Genetic Variation
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus / genetics
Humans
Influenza A Virus, H1N1 Subtype / genetics
Influenza A Virus, H1N1 Subtype / isolation & purification
Influenza A Virus, H3N2 Subtype / genetics
Influenza A Virus, H3N2 Subtype / isolation & purification
Influenza, Human / epidemiology
Influenza, Human / transmission*
Influenza, Human / virology
Male
Middle Aged
Orthomyxoviridae Infections / epidemiology
Orthomyxoviridae Infections / transmission
Orthomyxoviridae Infections / veterinary*
Orthomyxoviridae Infections / virology
Pandemics
Phylogeny
Swine / virology*
Swine Diseases / epidemiology
Swine Diseases / transmission*
Swine Diseases / virology
Thailand / epidemiology
Young Adult

Substances

Antibodies, Viral
H1N1 virus hemagglutinin
Hemagglutinin Glycoproteins, Influenza Virus