Abstract
Eight new eunicellin-base diterpenoids, hirsutalins A-H (1-8), were isolated from the soft coral Cladiella hirsuta. Their structures were elucidated by spectroscopic methods, particularly in 1D and 2D NMR experiments. The absolute configuration of 1 was determined by Mosher's method. Compounds 1, 5, and 6 have been shown to exhibit cytotoxicity toward several cancer cell lines. Compounds 2-4 and 8 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein, with compound 2 also effectively reducing the level of COX-2 protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anthozoa / chemistry*
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification*
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification*
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Antineoplastic Agents / pharmacology
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Cyclooxygenase 2 Inhibitors / chemistry
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Cyclooxygenase 2 Inhibitors / isolation & purification*
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Cyclooxygenase 2 Inhibitors / pharmacology
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Diterpenes / chemistry
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Diterpenes / isolation & purification*
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Diterpenes / pharmacology
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Drug Screening Assays, Antitumor
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Humans
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Lipopolysaccharides / pharmacology*
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Macrophages / drug effects*
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Mice
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Molecular Structure
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Nitric Oxide Synthase Type II / antagonists & inhibitors
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Nuclear Magnetic Resonance, Biomolecular
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Antineoplastic Agents
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Cyclooxygenase 2 Inhibitors
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Diterpenes
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Lipopolysaccharides
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eunicellin
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Nitric Oxide Synthase Type II