Abstract
To address the impact of partial reduction of Pten on tumor initiation, we generated PtenshRNA mice, in which PTEN expression was reduced below normal levels in various tissues. PtenshRNA mice frequently developed lymphoid and prostatic hyperplasia, splenomegaly, and sebaceous adenomas. Our observations support the notion that partial reduction of the dose of Pten with shRNA is sufficient to induce neoplastic disease in multiple organ systems.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adenoma / enzymology
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Adenoma / genetics
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Adrenal Glands / pathology
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Animals
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Brain / anatomy & histology
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Brain / enzymology
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Endometrium / pathology
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Female
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Hyperplasia
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Lymphatic Diseases / enzymology
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Lymphatic Diseases / genetics
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Neoplasms, Experimental / enzymology*
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Neoplasms, Experimental / genetics
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Organ Size
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PTEN Phosphohydrolase / biosynthesis
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PTEN Phosphohydrolase / deficiency*
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PTEN Phosphohydrolase / genetics*
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Phosphorylation
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Prostatic Hyperplasia / enzymology
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Prostatic Hyperplasia / genetics
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Prostatic Neoplasms / enzymology
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Prostatic Neoplasms / genetics
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Proto-Oncogene Proteins c-akt / metabolism
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RNA, Small Interfering / administration & dosage*
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RNA, Small Interfering / genetics
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Sebaceous Gland Neoplasms / enzymology
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Sebaceous Gland Neoplasms / genetics
Substances
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RNA, Small Interfering
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Proto-Oncogene Proteins c-akt
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PTEN Phosphohydrolase