Vascular Endothelial Growth Factor A isoform mRNA expression in pediatric acute myeloid leukemia

Pediatr Blood Cancer. 2011 Feb;56(2):294-7. doi: 10.1002/pbc.22783.

Abstract

In AML high VEGFA protein expression correlates with poor overall and relapse-free survival (OS/RFS). To date, the relevance of the various VEGFA isoforms is unclear. We determined VEGF121, VEGF145, VEGF148, VEGF165, VEGF183, and VEGF189 mRNA expression in pediatric AML samples and investigated the relation between VEGFA isoform expression and clinicopatholologic characteristics and outcome. A significant co-expression of VEGF121, VEGF165, VEGF183, and VEGF189 isoforms was apparent (mean rho = 0.716, P < 0.0001). This co-expression justifies measuring a single VEGFA isoform (e.g., 121, 165, 183, and 189) as representative expression of all VEGFA isoforms in future studies designed to determine the prognostic importance of VEGFA isoforms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Child
  • Child, Preschool
  • Disease-Free Survival
  • Female
  • Gene Expression
  • Gene Expression Profiling
  • Humans
  • Infant
  • Infant, Newborn
  • Kaplan-Meier Estimate
  • Leukemia, Myeloid, Acute / genetics
  • Leukemia, Myeloid, Acute / metabolism*
  • Leukemia, Myeloid, Acute / mortality
  • Male
  • Prognosis
  • Protein Isoforms / analysis
  • Protein Isoforms / biosynthesis
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Vascular Endothelial Growth Factor A / biosynthesis*
  • Vascular Endothelial Growth Factor A / genetics

Substances

  • Protein Isoforms
  • RNA, Messenger
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A