Laboratory-based rotavirus surveillance during the introduction of a vaccination program, Brazil, 2005-2009

Pediatr Infect Dis J. 2011 Jan;30(1 Suppl):S35-41. doi: 10.1097/INF.0b013e3181fefd5f.

Abstract

Background: : Brazil introduced universal antirotavirus vaccination in March 2006. This article reports the results of rotavirus A (RV-A) surveillance from January 2005 to December 2009.

Methods: : A total of 6109 fecal samples were collected in 18 Brazilian states. RV-A was detected by enzyme immunoassay and/or polyacrylamide gel electrophoresis, and genotyped through seminested reverse transcription-polymerase chain reaction.

Results: : RV-A was detected in 20.3% (n = 1242) of the samples. Among children less than 2 years old, regardless the antirotavirus vaccination status, the rates of RV-A detection were 33.8% in 2005, 23.7% in 2006, 16.8% in 2007, 22.9% in 2008, and 18.3% in 2009 (P < 0.001; χ test for linear trend). Among RV-A-positive samples, genotype G1P[8] or G1P[not typed(NT)] was detected in 14% in 2005, 12.3% in 2006, 9.5% in 2007, 0.7% in 2008, and 20.4% in 2009; G2P[4]/G2P[NT] was characterized in 9% in 2005, 49% in 2006, 66% in 2007, 85% in 2008, and 37.5% in 2009; G3P[8]/G3P[NT] was observed in 8.7% in 2005, 3.5% in 2006, and 5.7% in 2009; G9P[8]/G9P[NT] was observed in 52% in 2005, 22% in 2006, 12.3% in 2007, 3.2% in 2008, and 3.4% in 2009.

Conclusions: : Our data demonstrate the reemergence of RV-A genotype G2P[4] in Brazil from 2005 to 2008, and that the rate of G2P[4] detection decreased in 2009, probably reflecting natural oscillations of RV-A genotypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Brazil / epidemiology
  • Child
  • Child, Preschool
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay / methods
  • Feces / virology*
  • Female
  • Genotype
  • Humans
  • Immunization Programs
  • Infant
  • Infant, Newborn
  • Male
  • Middle Aged
  • Molecular Epidemiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / classification
  • Rotavirus / genetics
  • Rotavirus / isolation & purification*
  • Rotavirus Infections / epidemiology*
  • Rotavirus Infections / prevention & control*
  • Rotavirus Infections / virology
  • Rotavirus Vaccines / administration & dosage
  • Rotavirus Vaccines / immunology*
  • Young Adult

Substances

  • Rotavirus Vaccines