c-fos and c-myc expression in human endothelial cells as a function of different culture conditions

M G Lampugnani; N Polentarutti; M Pedenovi; A Mantovani; E Dejana; F Colotta

doi:10.1016/0014-4827(90)90320-a

c-fos and c-myc expression in human endothelial cells as a function of different culture conditions

Exp Cell Res. 1990 Feb;186(2):381-4. doi: 10.1016/0014-4827(90)90320-a.

Authors

M G Lampugnani¹, N Polentarutti, M Pedenovi, A Mantovani, E Dejana, F Colotta

Affiliation

¹ Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.

PMID: 2105224
DOI: 10.1016/0014-4827(90)90320-a

Abstract

Cultured human umbilical vein endothelial cells (HEC) could be induced to express c-fos and c-myc mRNA by either serum or ECGS (endothelial cell growth supplement). Neither agonist separately could support HEC proliferation but the combination did. Expression of c-fos and c-myc mRNA in the presence of both serum and ECGS was similar to that observed after each of the two stimuli was introduced separately. c-fos and c-myc expression in cultured HEC, even if related, is not necessarily accompanied by stimulation of cell growth.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood*
Cell Division
Cells, Cultured
Culture Media
Endothelial Growth Factors
Endothelium, Vascular / cytology
Endothelium, Vascular / metabolism*
Gene Expression*
Growth Substances / pharmacology*
Humans
Kinetics
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-fos
Proto-Oncogene Proteins c-myc
RNA, Messenger / genetics
Umbilical Veins

Substances

Culture Media
Endothelial Growth Factors
Growth Substances
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-fos
Proto-Oncogene Proteins c-myc
RNA, Messenger