Abstract
The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alkylation or arylation reaction.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Alkylation
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Calmodulin / chemistry*
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Calmodulin / metabolism
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Cysteine / chemistry*
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Cysteine / metabolism
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism
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Humans
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Nitriles / chemistry*
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Nitriles / metabolism
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Proteins / chemistry
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Proteins / metabolism
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Spectrophotometry, Infrared / methods
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Vibration
Substances
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CALML3 protein, human
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Calmodulin
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Fluorescent Dyes
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Nitriles
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Proteins
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Cysteine