Targeting the choroid plexus-CSF-brain nexus using peptides identified by phage display

Methods Mol Biol. 2011:686:483-98. doi: 10.1007/978-1-60761-938-3_25.

Abstract

Drug delivery to the central nervous system requires the use of specific portals to enable drug entry into the brain and, as such, there is a growing need to identify processes that can enable drug transfer across both blood-brain and blood-cerebrospinal fluid barriers. Phage display is a powerful combinatorial technique that identifies specific peptides that can confer new activities to inactive particles. Identification of these peptides is directly dependent on the specific screening strategies used for their selection and retrieval. This chapter describes three selection strategies, which can be used to identify peptides that target the choroid plexus (CP) directly or for drug translocation across the CP and into cerebrospinal fluid.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood-Brain Barrier / drug effects
  • Blood-Brain Barrier / physiology
  • Brain / blood supply
  • Brain / drug effects*
  • Brain / metabolism
  • Cerebrospinal Fluid / drug effects*
  • Cerebrospinal Fluid / metabolism
  • Choroid Plexus / drug effects*
  • Choroid Plexus / metabolism
  • Drug Delivery Systems
  • Mice
  • Mice, Inbred BALB C
  • Peptide Library*
  • Peptides / chemistry
  • Peptides / pharmacology*

Substances

  • Peptide Library
  • Peptides