Automated sample preparation for regulated bioanalysis: an integrated multiple assay extraction platform using robotic liquid handling

Bioanalysis. 2010 Jun;2(6):1023-40. doi: 10.4155/bio.10.55.

Abstract

Background: A novel approach for regulated bioanalytical sample preparation has been developed to combine multiple types of extraction techniques into one integrated and automated sample-preparation suite that pairs a graphical user interface with the Hamilton Microlab(®) STAR robotic liquid handler.

Results: The multi-assay sample-preparation suite is composed of three bioanalytical extraction techniques: protein precipitation, solid-phase extraction and liquid-liquid extraction. Validation data provided highly reproducible and robust results for each respective automated extraction technique.

Conclusion: The user-friendly graphical user interface and modular method design provide a flexible and versatile approach for routine bioanalytical sample-preparation and is the first fully integrated multiple assay sample-preparation suite for regulated bioanalysis.

MeSH terms

  • Analytic Sample Preparation Methods / instrumentation
  • Analytic Sample Preparation Methods / methods*
  • Automation
  • Chemical Fractionation / instrumentation
  • Chemical Fractionation / methods*
  • Chemical Precipitation
  • Computer Graphics
  • Drug Discovery
  • Humans
  • Pharmaceutical Preparations / analysis
  • Pharmaceutical Preparations / isolation & purification
  • Proteins / chemistry
  • Robotics*
  • Solid Phase Extraction
  • Systems Integration*
  • User-Computer Interface

Substances

  • Pharmaceutical Preparations
  • Proteins