Islet isolation from human pancreas stored in UW solution for 6 to 26 hours

Transplant Proc. 1990 Apr;22(2):763-4.

Abstract

1. Viable and functional islets can be recovered from human pancreata stored for prolonged periods (up to 18 hours) with either the UW solution or EC. 2. Function of islets from pancreas stored in UW solution appeared superior. However, the difference in these preliminary studies does not appear nearly so dramatic as in transplantation of vascularized pancreas. 3. Function of islets from human pancreas stored for longer than 18 hours after UW in situ flush was uniformly poor. 4. Considerable room for improvement in the storage of human pancreas before isolation exists. Progress in this area will be important for the development of any large-scale program of clinical islet transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine
  • Allopurinol
  • Animals
  • Blood Glucose / metabolism
  • Cell Separation / methods
  • Diabetes Mellitus, Experimental / blood
  • Diabetes Mellitus, Experimental / surgery*
  • Ficoll
  • Glutathione
  • Humans
  • Insulin
  • Islets of Langerhans / cytology
  • Islets of Langerhans / pathology
  • Islets of Langerhans Transplantation*
  • Mice
  • Mice, Nude
  • Organ Preservation Solutions*
  • Pancreas / cytology*
  • Pancreas / pathology
  • Raffinose
  • Solutions
  • Time Factors
  • Tissue Preservation

Substances

  • Blood Glucose
  • Insulin
  • Organ Preservation Solutions
  • Solutions
  • University of Wisconsin-lactobionate solution
  • Ficoll
  • Allopurinol
  • Glutathione
  • Adenosine
  • Raffinose