Shared molecular mechanisms regulate multiple catenin proteins: canonical Wnt signals and components modulate p120-catenin isoform-1 and additional p120 subfamily members

J Cell Sci. 2010 Dec 15;123(Pt 24):4351-65. doi: 10.1242/jcs.067199. Epub 2010 Nov 23.

Abstract

Wnt signaling pathways have fundamental roles in animal development and tumor progression. Here, employing Xenopus embryos and mammalian cell lines, we report that the degradation machinery of the canonical Wnt pathway modulates p120-catenin protein stability through mechanisms shared with those regulating β-catenin. For example, in common with β-catenin, exogenous expression of destruction complex components, such as GSK3β and axin, promotes degradation of p120-catenin. Again in parallel with β-catenin, reduction of canonical Wnt signals upon depletion of LRP5 and LRP6 results in p120-catenin degradation. At the primary sequence level, we resolved conserved GSK3β phosphorylation sites in the amino-terminal region of p120-catenin present exclusively in isoform-1. Point-mutagenesis of these residues inhibited the association of destruction complex components, such as those involved in ubiquitylation, resulting in stabilization of p120-catenin. Functionally, in line with predictions, p120 stabilization increased its signaling activity in the context of the p120-Kaiso pathway. Importantly, we found that two additional p120-catenin family members, ARVCF-catenin and δ-catenin, associate with axin and are degraded in its presence. Thus, as supported using gain- and loss-of-function approaches in embryo and cell line systems, canonical Wnt signals appear poised to have an impact upon a breadth of catenin biology in vertebrate development and, possibly, human cancers.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / metabolism
  • Amino Acid Sequence
  • Animals
  • Axin Protein
  • Casein Kinase I / metabolism
  • Catenins / chemistry
  • Catenins / metabolism*
  • Cell Line
  • Delta Catenin
  • Glycogen Synthase Kinase 3 / metabolism
  • Glycogen Synthase Kinase 3 beta
  • Humans
  • Molecular Sequence Data
  • Mutant Proteins / metabolism
  • Phosphorylation
  • Proteasome Endopeptidase Complex / metabolism
  • Protein Binding
  • Protein Interaction Mapping
  • Protein Isoforms / chemistry
  • Protein Isoforms / metabolism
  • Protein Processing, Post-Translational
  • Protein Stability
  • Protein Structure, Tertiary
  • Repressor Proteins / metabolism
  • Signal Transduction*
  • Ubiquitin / metabolism
  • Ubiquitination
  • Wnt Proteins / metabolism*
  • Xenopus
  • Xenopus Proteins

Substances

  • Adaptor Proteins, Signal Transducing
  • Axin Protein
  • Catenins
  • Mutant Proteins
  • Protein Isoforms
  • Repressor Proteins
  • Ubiquitin
  • Wnt Proteins
  • Xenopus Proteins
  • axin1 protein, Xenopus
  • Casein Kinase I
  • GSK3B protein, human
  • Glycogen Synthase Kinase 3 beta
  • Glycogen Synthase Kinase 3
  • Proteasome Endopeptidase Complex
  • Delta Catenin