Abstract
CD4(+)CD25(+)Foxp3(+) T-regulatory cells (Tregs) accumulate in tumors; however, little is known about how the tumor environment influences this process. Here we show that human melanomas express inducible T-cell costimulator ligand (ICOS-L/B7H) that can provide costimulation through ICOS for the expansion of activated Tregs maintaining high Foxp3 and CD25 expression as well as a suppressive function. Thus, ICOS-L expression by melanoma tumor cells may directly drive Treg activation and expansion in the tumor microenvironment as another mechanism of immune evasion.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD / genetics
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Antigens, CD / metabolism*
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Cell Line, Tumor
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Cell Proliferation*
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Coculture Techniques
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Flow Cytometry
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Forkhead Transcription Factors / metabolism
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Humans
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Inducible T-Cell Co-Stimulator Ligand
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Interferon-gamma / metabolism
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Interleukin-10 / metabolism
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Interleukin-2 Receptor alpha Subunit / metabolism
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L Cells
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Lymphocyte Activation / immunology
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Melanoma / genetics
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Melanoma / metabolism*
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Melanoma / pathology
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Melanoma, Experimental / genetics
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Melanoma, Experimental / metabolism
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Melanoma, Experimental / pathology
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Mice
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Mice, Inbred C57BL
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Reverse Transcriptase Polymerase Chain Reaction
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T-Lymphocytes, Regulatory / immunology
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T-Lymphocytes, Regulatory / metabolism*
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Tumor Microenvironment
Substances
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Antigens, CD
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FOXP3 protein, human
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Forkhead Transcription Factors
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ICOSLG protein, human
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Inducible T-Cell Co-Stimulator Ligand
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Interleukin-2 Receptor alpha Subunit
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Interleukin-10
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Interferon-gamma