Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges cytotoxin B cell test and culture as gold standard

J Clin Microbiol. 2011 Feb;49(2):710-1. doi: 10.1128/JCM.01824-10. Epub 2010 Nov 24.

Abstract

Compared to the composite gold standard cytotoxin B assay and toxigenic culture, the loop-mediated isothermal amplification (LAMP) test for Clostridium difficile had a sensitivity and specificity of 98%, positive predictive value of 92%, and negative predictive value of >99%. A one-hour turnaround time for the LAMP test provides rapid diagnosis and cost savings.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques / methods*
  • Clostridioides difficile / genetics
  • Clostridioides difficile / isolation & purification*
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • Enterocolitis, Pseudomembranous / diagnosis*
  • Enterocolitis, Pseudomembranous / microbiology
  • Humans
  • Nucleic Acid Amplification Techniques / methods*
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Time Factors

Substances

  • DNA, Bacterial