Revealing new measles virus transmission routes by use of sequence analysis of phosphoprotein and hemagglutinin genes

J Clin Microbiol. 2011 Feb;49(2):677-83. doi: 10.1128/JCM.01703-10. Epub 2010 Nov 24.

Abstract

With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-HVR sequences. However, these strains showed considerable diversity within a larger sequencing window based on concatenated MV phosphoprotein and hemagglutinin genes (P/H pseudogenes). In Belarus, Germany, Russia, and the Democratic Republic of Congo, the P/H pseudogenes provided insights into chains of transmission, whereas identical NP-HVR provided none. In Russia, for instance, the P/H pseudogene identified temporal clusters rather than geographical clusters, demonstrating the circulation and importation of independent variants rather than large local outbreaks lasting for several years, as suggested by NP-HVR. Thus, by extending the sequencing window for molecular epidemiology, a more refined picture of MV circulation was obtained with more clearly defined links between outbreaks and transmission chains. Our results also suggested that in contrast to the P gene, the H gene acquired fixed substitutions that continued to be found in subsequent outbreaks, possibly with consequences for its antigenicity. Thus, a longer sequencing window has true benefits both for the epidemiological surveillance of measles and for the better monitoring of viral evolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Africa / epidemiology
  • Cluster Analysis
  • Disease Outbreaks*
  • Europe / epidemiology
  • Hemagglutinins, Viral / genetics*
  • Humans
  • Measles / epidemiology*
  • Measles / transmission*
  • Measles / virology
  • Measles virus / classification*
  • Measles virus / genetics*
  • Measles virus / isolation & purification
  • Molecular Epidemiology
  • Molecular Sequence Data
  • Molecular Typing
  • Nucleocapsid Proteins
  • Nucleoproteins / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology
  • Viral Proteins / genetics*

Substances

  • Hemagglutinins, Viral
  • Nucleocapsid Proteins
  • Nucleoproteins
  • Viral Proteins
  • hemagglutinin protein G, measles virus
  • nucleoprotein, Measles virus

Associated data

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