Endogenous dopamine (DA) competes with the binding of a radiolabeled D₃ receptor partial agonist in vivo: a positron emission tomography study

Synapse. 2011 Aug;65(8):724-32. doi: 10.1002/syn.20891. Epub 2011 Mar 21.

Abstract

A series of microPET imaging studies were conducted in anesthetized rhesus monkeys using the dopamine D₃-selective partial agonist, [¹⁸F]5. There was variable uptake in regions of brain known to express a high density of D₃ receptors under baseline conditions. Pretreatment with lorazepam (1 mg/kg, i.v. 30 min) to reduce endogenous dopamine activity before tracer injection resulted in a dramatic increase in uptake in the caudate, putamen, and thalamus, and an increase in the binding potential (BP) values, a measure of D₃ receptor binding in vivo. These data indicate that there is a high level of competition between [¹⁸F]5 and endogenous dopamine for D₃ receptors in vivo.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Benzamides / chemical synthesis
  • Benzamides / pharmacokinetics
  • Binding, Competitive*
  • Brain / diagnostic imaging*
  • Brain / metabolism
  • Dopamine / pharmacokinetics*
  • Dopamine Agonists / chemical synthesis
  • Dopamine Agonists / pharmacokinetics*
  • Fluorine Radioisotopes / pharmacokinetics
  • Macaca mulatta
  • Male
  • Piperazines / chemical synthesis
  • Piperazines / pharmacokinetics
  • Positron-Emission Tomography
  • Radiopharmaceuticals / chemical synthesis
  • Radiopharmaceuticals / pharmacokinetics*
  • Receptors, Dopamine D3 / metabolism

Substances

  • Benzamides
  • Dopamine Agonists
  • Fluorine Radioisotopes
  • Piperazines
  • Radiopharmaceuticals
  • Receptors, Dopamine D3
  • Dopamine