Differentiation between Aδ and C fibre evoked nociceptive reflexes by TTX resistance and opioid sensitivity in the cat

Neurosci Res. 2011 Mar;69(3):241-5. doi: 10.1016/j.neures.2010.12.005. Epub 2010 Dec 13.

Abstract

The contribution of nociceptive Aδ-fibres and C-fibres of the central pad of the foot to nociceptive spinal flexor reflex pathways (FRA-type) and to nociceptive excitatory reflex pathways to foot extensors (non-FRA type) was investigated in high spinal cats. Persisting effects after complete blocking of A-fibres by tetrodotoxin (TTX) application were thus attributed to nociceptive C-fibres. The results revealed that both Aδ and C-fibre afferents contributed to nociceptive reflexes of a FRA-pattern and of a non-FRA pattern. Thereby, the effects of Aδ-fibres were evoked with a distinctly shorter delay than those of C-fibres. Furthermore, Aδ-fibres partly exerted a significant inhibitory influence on the C-fibre action in FRA and non-FRA pathways. Treatment with different opioid receptor agonists (DAMGO and DSLET) and subsequently with naloxone revealed that a distinct part of the opioid action on nociceptive reflex pathways of the FRA-type and of the non-FRA type was evidently exerted via Aδ-fibres.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analgesics / pharmacology
  • Analgesics, Opioid / pharmacology*
  • Anesthetics, Local / pharmacology
  • Animals
  • Cats
  • Dose-Response Relationship, Drug
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / pharmacology
  • Enkephalin, Leucine / analogs & derivatives
  • Enkephalin, Leucine / pharmacology
  • Excitatory Postsynaptic Potentials / drug effects
  • Female
  • Foot / innervation
  • Male
  • Naloxone / pharmacology
  • Narcotic Antagonists / pharmacology
  • Nerve Fibers, Myelinated / drug effects*
  • Nerve Fibers, Myelinated / physiology
  • Nerve Fibers, Unmyelinated / drug effects*
  • Nerve Fibers, Unmyelinated / physiology
  • Nociceptors / drug effects
  • Nociceptors / physiology*
  • Reflex / drug effects*
  • Spinal Cord / cytology*
  • Tetrodotoxin / pharmacology

Substances

  • Analgesics
  • Analgesics, Opioid
  • Anesthetics, Local
  • Narcotic Antagonists
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
  • Naloxone
  • Tetrodotoxin
  • Enkephalin, Leucine
  • enkephalin, Ser(2), Leu(5), Thr(6)-