Multi-parameter determination of TNFα, PCT and CRP for point-of-care testing

Analyst. 2011 Feb 21;136(4):692-5. doi: 10.1039/c0an00699h. Epub 2010 Dec 15.

Abstract

A proof of concept for the simultaneous multi-parameter determination of three inflammation and sepsis parameters-TNFα, PCT and CRP-using a compact optical immunosensor is demonstrated. Harmonized assay conditions revealed standard curves with test midpoints (IC(50)) of 380 µg L(-1) for TNFα, 2300 µg L(-1) for PCT, and 2645 µg L(-1) for CRP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / analysis
  • Biomarkers / chemistry
  • Biosensing Techniques / methods*
  • C-Reactive Protein / analysis*
  • C-Reactive Protein / chemistry
  • Calcitonin / analysis*
  • Calcitonin / chemistry
  • Humans
  • Immunoassay / methods*
  • Inflammation / metabolism
  • Lasers
  • Molecular Weight
  • Optical Phenomena
  • Point-of-Care Systems*
  • Protein Precursors / analysis*
  • Protein Precursors / chemistry
  • Tumor Necrosis Factor-alpha / analysis*
  • Tumor Necrosis Factor-alpha / chemistry

Substances

  • Biomarkers
  • Protein Precursors
  • Tumor Necrosis Factor-alpha
  • Calcitonin
  • C-Reactive Protein