Abstract
Old yellow enzyme (OYE) is an NADPH oxidoreductase which contains flavin mononucleotide as prosthetic group. The X-ray structures of OYE from Trypanosoma cruzi (TcOYE) which produces prostaglandin (PG) F(2α) from PGH(2) have been determined in the presence or absence of menadione. The binding motif of menadione, known as one of the inhibitors for TcOYE, should accelerate the structure-based development of novel anti-chagasic drugs that inhibit PGF(2α) production specifically.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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Crystallography, X-Ray
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Flavin Mononucleotide / chemistry
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Hydroxyprostaglandin Dehydrogenases / antagonists & inhibitors*
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Hydroxyprostaglandin Dehydrogenases / metabolism
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Models, Molecular
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NADPH Dehydrogenase / antagonists & inhibitors*
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NADPH Dehydrogenase / chemistry*
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Trypanosoma cruzi / enzymology*
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Vitamin K 3 / chemistry*
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Vitamin K 3 / pharmacology
Substances
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Vitamin K 3
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Flavin Mononucleotide
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Hydroxyprostaglandin Dehydrogenases
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prostaglandin-F synthase
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NADPH Dehydrogenase