Ring chromosome 22 and neurofibromatosis type II: proof of two-hit model for the loss of the NF2 gene in the development of meningioma

Clin Genet. 2012 Jan;81(1):82-7. doi: 10.1111/j.1399-0004.2010.01598.x. Epub 2010 Dec 22.

Abstract

Carriers of a ring chromosome 22 are mentally retarded and show variable facial dysmorphism. They may also present with features of neurofibromatosis type II (NF2) such as vestibular schwannomas and multiple meningiomas. In these cases, tumourigenesis has been suspected to be caused by the loss of both alleles of the NF2 gene, a tumour suppressor localized in 22q12.2. Here, we describe an 18-year-old patient with constitutional ring chromosome 22 and mental retardation who developed rapid-onset spastic paraparesis at the age of 15 years. The causative spinal meningioma at the level of T3, which compressed the spinal cord, was surgically removed, and the patient regained ambulation. Array comparative genomic hybridization (array CGH) and multiplex ligation-dependent probe amplification (MLPA) analyses in blood revealed a terminal deletion in 22q13.32, not comprising the NF2 gene. In tumour tissue, loss of the whole ring chromosome 22 including one NF2 gene due to mitotic instability constituted the likely first hit, while a point mutation in the other allele of the NF2 gene (c.784C>T, p.R262X) was shown as second hit. We review all cases from the literature and suggest clinical guidelines for surveillance of patients with ring chromosome 22.

Publication types

  • Case Reports

MeSH terms

  • Adolescent
  • Alleles
  • Chromosome Deletion*
  • Chromosomes, Human, Pair 22 / genetics*
  • Comparative Genomic Hybridization
  • Genes, Neurofibromatosis 2*
  • Genetic Testing / standards
  • Genomic Instability
  • Humans
  • Magnetic Resonance Imaging
  • Male
  • Meningioma / genetics*
  • Meningioma / surgery
  • Models, Genetic
  • Neurofibromatosis 2 / genetics*
  • Point Mutation
  • Ring Chromosomes*