Human glucose-6-phosphate dehydrogenase gene carried on a yeast artificial chromosome encodes active enzyme in monkey cells

M D'Urso; I Zucchi; A Ciccodicola; G Palmieri; F E Abidi; D Schlessinger

doi:10.1016/0888-7543(90)90196-2

Human glucose-6-phosphate dehydrogenase gene carried on a yeast artificial chromosome encodes active enzyme in monkey cells

Genomics. 1990 Aug;7(4):531-4. doi: 10.1016/0888-7543(90)90196-2.

Authors

M D'Urso¹, I Zucchi, A Ciccodicola, G Palmieri, F E Abidi, D Schlessinger

Affiliation

¹ International Institute of Genetics and Biophysics, Naples, Italy.

PMID: 2117564
DOI: 10.1016/0888-7543(90)90196-2

Abstract

Yeast artificial chromosomes (YACs) permit the cloning of large tracts of human DNA. A YAC containing the human glucose-6-phosphate dehydrogenase gene is shown to encode active enzyme, supporting the inference that the YAC conserves the structure of the genomic DNA.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Blotting, Southern
Chromosomes, Fungal
Cloning, Molecular*
Gene Expression
Gene Library
Genes
Genetic Vectors
Genome, Human
Guanine Nucleotides / genetics*
Guanosine Diphosphate / genetics*
Guanosine Diphosphate / metabolism
Haplorhini
Humans
Hybrid Cells
Restriction Mapping

Substances

Guanine Nucleotides
Guanosine Diphosphate