Abstract
A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques / methods*
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Humans
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Minisatellite Repeats*
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Mycobacterium / classification*
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Mycobacterium / genetics*
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Mycobacterium / isolation & purification
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Mycobacterium Infections / microbiology
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity