Abstract
Manganese has an important yet undefined role in the virulence of many bacterial pathogens. In this study we confirm that a null mutation in Yersinia pseudotuberculosis mntH reduces intracellular manganese accumulation. An mntH mutant was susceptible to killing by reactive oxygen species when grown under manganese-limited conditions. The mntH mutant was defective in survival and growth in macrophages expressing functional Nramp1, but in macrophages deficient in Nramp the bacteria were able to survive and replicate. In Galleria mellonella, the mntH mutant was attenuated. Taken together, these data suggest a role for manganese in Y. pseudotuberculosis during macrophage intracellular survival, protecting the bacteria from the antimicrobial products released during the respiratory burst.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Cation Transport Proteins / genetics
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Cation Transport Proteins / metabolism*
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Cell Line
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Gene Knockout Techniques
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Genetic Complementation Test
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Lepidoptera / microbiology
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Macrophages / microbiology*
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Manganese / metabolism*
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Mice
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Microbial Viability*
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Oxidative Stress
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Reactive Oxygen Species / toxicity
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Survival Analysis
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Virulence
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Yersinia pseudotuberculosis / drug effects
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Yersinia pseudotuberculosis / genetics
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Yersinia pseudotuberculosis / metabolism
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Yersinia pseudotuberculosis / physiology*
Substances
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Bacterial Proteins
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Cation Transport Proteins
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MntH protein, bacteria
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Reactive Oxygen Species
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natural resistance-associated macrophage protein 1
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Manganese