Genetic analysis of the xenobiotic resistance-associated ABC gene subfamilies of the Lepidoptera

Insect Mol Biol. 2011 Apr;20(2):243-56. doi: 10.1111/j.1365-2583.2010.01064.x. Epub 2010 Dec 27.

Abstract

Some ATP-binding cassette (ABC) transporters of subfamilies B, C and G confer resistance to xenobiotics including insecticides. We identified genes of these subfamilies expressed by the lepidopterans Trichoplusia ni and Bombyx mori. The B. mori genome includes eight, six and 13 ABC-B, -C and -G genes, respectively, which encode P-glycoprotein, multidrug resistance protein, MRP, and breast cancer resistance protein, BCRP, homologues. Among the ABC-C and -G subfamilies, gene duplication contributes to protein diversity. We have identified three ABC-B and two ABC-C T. ni genes. Analyses of the T. ni MRP (TrnMRP) revealed unique features, including the potential for TrnMRP4 hyperglycosylation and the alternative splicing of TrnMRP1. Taken together, these attributes of moth multidrug resistance-associated ABCs may confer distinct functional capacities to xenobiotic efflux.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B / genetics
  • ATP-Binding Cassette Transporters / genetics*
  • Alternative Splicing
  • Animals
  • Base Sequence
  • Bombyx / genetics*
  • Gene Duplication
  • Genetic Variation
  • Insect Proteins / genetics*
  • Molecular Sequence Data
  • Moths / genetics*
  • Multidrug Resistance-Associated Proteins / genetics
  • Phylogeny
  • Sequence Alignment

Substances

  • ATP Binding Cassette Transporter, Subfamily B
  • ATP-Binding Cassette Transporters
  • Insect Proteins
  • Multidrug Resistance-Associated Proteins