Measurement of exhaled nitric oxide as a potential screening tool for pulmonary tuberculosis

Int J Tuberc Lung Dis. 2011 Feb;15(2):185-92.

Abstract

Background: There is a need for low-technology, inexpensive screening tools for active tuberculosis (TB) case finding.

Objective: to assess the potential usefulness of measuring exhaled nitric oxide (eNO).

Design: Cross-sectional comparison in Hanoi, Viet Nam, comparing 90 consecutive smear-positive, culture-confirmed TB patients presenting at a referral hospital with office workers (no X-ray confirming TB) at this hospital (n = 52) and at a construction firm (n = 84). eNO levels were analysed using a validated handheld analyser.

Results: eNO levels among TB patients (median 15 parts per billion [ppb], interquartile range [IQR] 10-20) were equal to those among construction firm workers (15 ppb, IQR 12-19, P = 0.517) but higher than those among hospital workers (8.5 ppb, IQR 5-12.5, P < 0.001). Taking the hospital workers as the comparison group, best performance as a diagnostic tool was at a cut-off of 10 ppb, with sensitivity 78% (95%CI 68-86) and specificity 62% (95%CI 47-75). Test characteristics could be optimised to 84% vs. 67% by excluding individuals who had recently smoked or consumed alcohol.

Conclusion: While eNO measurement has limited value in the direct diagnosis of pulmonary TB, it may be worth developing and evaluating as a cost-effective replacement of chest X-ray in screening algorithms of pulmonary TB where X-ray is not available.

Publication types

  • Evaluation Study
  • Multicenter Study

MeSH terms

  • Adult
  • Biomarkers / analysis
  • Breath Tests*
  • Case-Control Studies
  • Chi-Square Distribution
  • Cross-Sectional Studies
  • Female
  • Humans
  • Linear Models
  • Logistic Models
  • Male
  • Mass Screening / methods*
  • Middle Aged
  • Mycobacterium tuberculosis / isolation & purification
  • Netherlands
  • Nitric Oxide / analysis*
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Sputum / microbiology
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / metabolism
  • Tuberculosis, Pulmonary / microbiology
  • Tuberculosis, Pulmonary / physiopathology
  • Vietnam
  • Young Adult

Substances

  • Biomarkers
  • Nitric Oxide