Abstract
A pseudotype baculovirus with the glycoprotein of vesicular stomatitis virus (VSV-G) on the envelope was used as a vector for the construction of recombinant baculovirus expressing the G protein of rabies virus (RABV) under the cytomegalovirus (CMV) promoter. The generated recombinant baculovirus (BV-G) efficiently expressed the RABV G proteins in mammalian cells. Intramuscular vaccination with BV-G (10(9) PFU/mouse) induced the production of RABV G-specific neutralizing antibodies and strong T cell responses in mice. Our data clearly indicate that pseudotype baculovirus-mediated gene delivery can be utilized as an alternative strategy to develop a new generation of vaccine against RABV infection.
MeSH terms
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Animals
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Antibodies, Neutralizing / blood
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Antibodies, Viral / blood
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Antigens, Viral / genetics
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Antigens, Viral / immunology*
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Baculoviridae / genetics*
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Drug Carriers*
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Female
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Genetic Vectors*
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Glycoproteins / genetics
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Glycoproteins / immunology*
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Injections, Intramuscular
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Mice
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Mice, Inbred ICR
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Rabies Vaccines / administration & dosage
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Rabies Vaccines / genetics
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Rabies Vaccines / immunology*
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Rabies virus / genetics
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Rabies virus / immunology*
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Vaccination / methods
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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Vesiculovirus / genetics*
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
Substances
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Antibodies, Neutralizing
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Antibodies, Viral
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Antigens, Viral
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Drug Carriers
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Glycoproteins
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Rabies Vaccines
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Vaccines, Synthetic
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Viral Envelope Proteins
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glycoprotein G, Rabies virus