Diagnosis of anal human papillomavirus infection: polymerase chain reaction or cytology?

Int J Infect Dis. 2011 Apr;15(4):e232-5. doi: 10.1016/j.ijid.2010.11.012. Epub 2011 Jan 15.

Abstract

Objectives: To investigate the relationship between human immunodeficiency virus (HIV)-positive and HIV-negative patients engaging in promiscuous behaviors and anal human papillomavirus (HPV) infection diagnosed by polymerase chain reaction (PCR) and cytology.

Methods: Fifty-six HIV-positive patients and 49 HIV-negative patients who engaged in sexually promiscuous behavior were enrolled in the study. We performed cytological exams using the Pap smear and PCR for HPV-DNA detection, with identification of oncogenic strains. The 2001 Bethesda System terminology was used for the cytological exams. We also evaluated the immunologic status of the HIV-infected patients.

Results: PCR positivity for HPV-DNA was higher in the group of HIV-positive patients than in the group of HIV-negative patients with a statistically significant difference. In contrast we did not find any statistically significant difference by cytological exam. Oncogenic strains were equally distributed in the two groups.

Conclusions: Our results indicate the importance of the cytological exam for anal HPV screening in the population at high risk of sexually transmitted disease and that HPV-DNA PCR can be used only as adjunct test.

Publication types

  • Evaluation Study

MeSH terms

  • AIDS-Related Opportunistic Infections / diagnosis
  • AIDS-Related Opportunistic Infections / epidemiology
  • AIDS-Related Opportunistic Infections / pathology
  • AIDS-Related Opportunistic Infections / virology
  • Adult
  • Aged
  • Anal Canal / pathology*
  • Anal Canal / virology
  • Anus Diseases / diagnosis*
  • Anus Diseases / epidemiology
  • Anus Diseases / pathology*
  • Anus Diseases / virology
  • Cytodiagnosis / methods*
  • DNA, Viral / analysis
  • Female
  • HIV Infections / complications*
  • Humans
  • Male
  • Middle Aged
  • Papanicolaou Test
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / epidemiology
  • Papillomavirus Infections / pathology*
  • Papillomavirus Infections / virology
  • Polymerase Chain Reaction / methods*
  • Risk-Taking
  • Sexual Behavior
  • Vaginal Smears
  • Young Adult

Substances

  • DNA, Viral