Preparation and characterization of a three-dimensional printed scaffold based on a functionalized polyester for bone tissue engineering applications

Acta Biomater. 2011 May;7(5):1999-2006. doi: 10.1016/j.actbio.2011.01.018. Epub 2011 Jan 15.

Abstract

At present there is a strong need for suitable scaffolds that meet the requirements for bone tissue engineering applications. The objective of this study was to investigate the suitability of porous scaffolds based on a hydroxyl functionalized polymer, poly(hydroxymethylglycolide-co-ε-caprolactone) (pHMGCL), for tissue engineering. In a recent study this polymer was shown to be a promising material for bone regeneration. The scaffolds consisting of pHMGCL or poly(ε-caprolactone) (PCL) were produced by means of a rapid prototyping technique (three-dimensional plotting) and were shown to have a high porosity and an interconnected pore structure. The thermal and mechanical properties of both scaffolds were investigated and human mesenchymal stem cells were seeded onto the scaffolds to evaluate the cell attachment properties, as well as cell viability and differentiation. It was shown that the cells filled the pores of the pHMGCL scaffold within 7 days and displayed increased metabolic activity when compared with cells cultured in PCL scaffolds. Importantly, pHMGCL scaffolds supported osteogenic differentiation. Therefore, scaffolds based on pHMGCL are promising templates for bone tissue engineering applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone and Bones / drug effects
  • Bone and Bones / physiology*
  • Cell Adhesion / drug effects
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Humans
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / metabolism
  • Osteogenesis / drug effects
  • Polyesters / chemical synthesis
  • Polyesters / chemistry*
  • Polyesters / pharmacology
  • Tissue Engineering / methods*
  • Tissue Scaffolds / chemistry*

Substances

  • Polyesters
  • polycaprolactone