Growth differentiation factor 15 deficiency protects against atherosclerosis by attenuating CCR2-mediated macrophage chemotaxis

J Exp Med. 2011 Feb 14;208(2):217-25. doi: 10.1084/jem.20100370. Epub 2011 Jan 17.

Abstract

Growth differentiation factor (GDF) 15 is a member of the transforming growth factor β (TGF-β) superfamily, which operates in acute phase responses through a currently unknown receptor. Elevated GDF-15 serum levels were recently identified as a risk factor for acute coronary syndromes. We show that GDF-15 expression is up-regulated as disease progresses in murine atherosclerosis and primarily colocalizes with plaque macrophages. Hematopoietic GDF-15 deficiency in low density lipoprotein receptor(-/-) mice led to impaired initial lesion formation and increased collagen in later lesions. Although lesion burden in GDF-15(-/-) chimeras was unaltered, plaques had reduced macrophage infiltrates and decreased necrotic core formation, all features of improved plaque stability. In vitro studies pointed to a TGFβRII-dependent regulatory role of GDF-15 in cell death regulation. Importantly, GDF-15(-/-) macrophages displayed reduced CCR2 expression, whereas GDF-15 promoted macrophage chemotaxis in a strictly CCR2- and TGFβRII-dependent manner, a phenomenon which was not observed in G protein-coupled receptor kinase 2(+/-) macrophages. In conclusion, GDF-15 deletion has a beneficial effect both in early and later atherosclerosis by inhibition of CCR2-mediated chemotaxis and by modulating cell death. Our study is the first to identify GDF-15 as an acute phase modifier of CCR2/TGFβRII-dependent inflammatory responses to vascular injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Atherosclerosis / prevention & control*
  • Bone Marrow Transplantation
  • Chemotaxis / physiology*
  • DNA Primers / genetics
  • Flow Cytometry
  • Gene Expression Regulation / physiology*
  • Growth Differentiation Factor 15 / blood
  • Growth Differentiation Factor 15 / deficiency*
  • Growth Differentiation Factor 15 / genetics
  • Immunohistochemistry
  • Macrophages / physiology*
  • Mice
  • Mice, Knockout
  • Phagocytosis / physiology
  • Receptors, CCR2 / metabolism*
  • Receptors, LDL / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Ccr2 protein, mouse
  • DNA Primers
  • Gdf15 protein, mouse
  • Growth Differentiation Factor 15
  • Receptors, CCR2
  • Receptors, LDL