Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development

Seiichi Koike; Yoshifumi Yutoh; Kazuko Keino-Masu; Sumihare Noji; Masayuki Masu; Hideyo Ohuchi

doi:10.1002/dvdy.22543

Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development

Dev Dyn. 2011 Feb;240(2):413-21. doi: 10.1002/dvdy.22543. Epub 2011 Jan 11.

Authors

Seiichi Koike¹, Yoshifumi Yutoh, Kazuko Keino-Masu, Sumihare Noji, Masayuki Masu, Hideyo Ohuchi

Affiliation

¹ Department of Molecular Neurobiology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.

PMID: 21246658
DOI: 10.1002/dvdy.22543

Abstract

Autotaxin (ATX) is a lysophospholipid-generating exoenzyme expressed in embryonic and adult neural tissues. We previously showed that ATX is expressed in the neural organizing centers, anterior head process, and midbrain-hindbrain boundary (MHB). To elucidate the role of ATX during neural development, here we examined the neural phenotypes of ATX-deficient mice. Expression analysis of neural marker genes revealed that lateral expansion of the rostral forebrain is reduced and establishment of the MHB is compromised as early as the late headfold stage in ATX mutant embryos. Moreover, ATX mutant embryos fail to complete cranial neural tube closure. These results indicate that ATX is essential for cranial neurulation and MHB establishment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biomarkers / metabolism
Embryo, Mammalian / anatomy & histology
Embryo, Mammalian / physiology
Eye Proteins / genetics
Eye Proteins / metabolism
Fibroblast Growth Factor 8 / genetics
Fibroblast Growth Factor 8 / metabolism
Gene Expression Regulation, Developmental
Homeobox Protein SIX3
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism
In Situ Hybridization
Mesencephalon / anatomy & histology
Mesencephalon / embryology*
Mice
Mice, Knockout
Multienzyme Complexes / genetics
Multienzyme Complexes / metabolism*
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Neurulation / physiology*
Otx Transcription Factors / genetics
Otx Transcription Factors / metabolism
Phosphodiesterase I / genetics
Phosphodiesterase I / metabolism*
Phosphoric Diester Hydrolases
Pyrophosphatases / genetics
Pyrophosphatases / metabolism*
Rhombencephalon / anatomy & histology
Rhombencephalon / embryology*

Substances

Biomarkers
Eye Proteins
Fgf8 protein, mouse
Homeodomain Proteins
Multienzyme Complexes
Nerve Tissue Proteins
Otx Transcription Factors
Otx2 protein, mouse
Fibroblast Growth Factor 8
Phosphoric Diester Hydrolases
Phosphodiesterase I
alkylglycerophosphoethanolamine phosphodiesterase
Pyrophosphatases