Six hundred children from urban and rural regions of Hadhramout governorate were examined targeted for the detection of intestinal parasites during the year 2009. The main infective parasites prevailed in children were Gardia lamblia (19.17%), Entamoeba histolytica (16.83%), Ascaris lumbricoides (15.83%), Trichuris trichiura (2.33%), Hymenolepis nana (2.33%), Taenia saginata (1.50%) and Schistosoma mansoni (0.67%). Besides, infections were accompanied by different symptoms as diarrhea (43.5%), abdominal pain (23.3%), abdominal distention (17.3%), constipation (9.1%), nausea and vomiting (8.02%) and fever (5.1%). The parasitosis reflected the hygienic problems and their influence on public health of Hadhramout.