Abstract
Peptide hormones and neuropeptides are packaged and stored in a specialized intracellular organelle called the dense core vesicle. It remains elusive how peptide cargoes are correctly sorted. In the present study, we show that a highly conserved Golgi-localized protein named HID-1 acts to prevent mis-sorting of peptide cargoes to lysosomes for degradation via a PtdIns3P-dependent trafficking pathway. Epistasis analysis suggests that rab-2 is epistatic to hid-1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caenorhabditis elegans
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism
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Caenorhabditis elegans Proteins / physiology*
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Exocytosis
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Golgi Apparatus / metabolism
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Lysosomes / metabolism
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Membrane Glycoproteins / metabolism
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Mutation
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Neuropeptides / metabolism*
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Protein Transport
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Protein Tyrosine Phosphatases / metabolism
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Secretory Vesicles / metabolism
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Vesicular Transport Proteins / genetics
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Vesicular Transport Proteins / physiology*
Substances
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Caenorhabditis elegans Proteins
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HID-1 protein, C elegans
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Membrane Glycoproteins
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Neuropeptides
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Vesicular Transport Proteins
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IDA-1 protein, C elegans
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Protein Tyrosine Phosphatases