Abstract
We characterized 17 clinical isolates of Klebsiella pneumoniae producing 16S rRNA methylase ArmA. The isolates originated in Poland from 2002 to May 2010 and encompassed four XbaI-PFGE clusters. All the isolates were resistant to amikacin, gentamicin and kanamycin (MIC range: 256-1024 mg l(-1)) and carried the armA gene on a large plasmid of approximately 90 or 130 kb in 15 and 2 isolates, respectively. The armA gene was found in a ~10 kb ClaI restriction fragment of the large plasmid and was flanked by the same elements as in Tn1548. All the isolates carried the bla(CTX-M) gene for a CTX-M-type extended-spectrum β-lactamase. Our results show that ArmA has disseminated horizontally among K. pneumoniae isolates in Poland on the ~90 kb plasmid of the pCTX-M3 family.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminoglycosides / pharmacology*
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Anti-Bacterial Agents / pharmacology*
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DNA Transposable Elements
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial*
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Gene Transfer, Horizontal
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Humans
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Klebsiella Infections / microbiology*
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Klebsiella pneumoniae / enzymology*
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Methylation
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Methyltransferases / biosynthesis*
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Methyltransferases / genetics
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Microbial Sensitivity Tests
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Plasmids
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Poland
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RNA, Bacterial / metabolism
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RNA, Ribosomal, 16S / metabolism
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beta-Lactamases / genetics
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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DNA Transposable Elements
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DNA, Bacterial
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RNA, Bacterial
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RNA, Ribosomal, 16S
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Methyltransferases
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rRNA (adenosine-O-2'-)methyltransferase
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beta-Lactamases