Abstract
A series of expression cassettes which mediate secretion or surface display of antibody fragments was stably integrated in the chromosome of Lactobacillus paracasei. L. paracasei producing surface-anchored variable domain of llama heavy chain (VHH) (ARP1) directed against rotavirus showed efficient binding to rotavirus and protection in the mouse model of rotavirus infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology
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Enzyme-Linked Immunosorbent Assay
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Immunoglobulin Fragments / genetics*
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Immunoglobulin Fragments / physiology
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Lactobacillus / genetics*
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Limosilactobacillus fermentum / genetics
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Limosilactobacillus fermentum / immunology
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Mice
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Rotavirus Infections / immunology
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Rotavirus Infections / therapy*
Substances
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APF protein, Lactobacillus crispatus
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Bacterial Proteins
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Immunoglobulin Fragments