Objective: To explore the changes of Muc5ac expression in a rat model of mucus hypersecretion induced by acrolein.
Methods: The rat model of mucus hypersceretion was established by acrolein exposure. RT-PCR, ISH, immunohistochemistry and Western blot were employed to detect the mRNA and protein levels of Muc5ac in the rats airway.
Results: The expression of Muc5ac was detected in trachea and lung at 3 weeks post exposure (wpe) and the expression level was enhanced at 6 wpe. ISH confirmed that Muc5ac was synthesized endogenously.
Conclusion: The pulmonary mucus hypersecretion is associated to the increase of intrinsic Muc5ac expression in the rat airway exposure to acrolein.