A facile method to increase titers of miRNA-encoding retroviruses by inhibition of the RNaseIII enzyme Drosha

Eur J Immunol. 2011 Feb;41(2):549-51. doi: 10.1002/eji.201040960. Epub 2010 Dec 29.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / metabolism
  • B-Lymphocytes / virology
  • Genes, Reporter / genetics
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism*
  • Mice
  • MicroRNAs / genetics*
  • NIH 3T3 Cells
  • RNA, Small Interfering / genetics
  • Retroviridae / genetics*
  • Ribonuclease III / antagonists & inhibitors*
  • Ribonuclease III / genetics
  • Ribonuclease III / metabolism
  • Transduction, Genetic / methods*
  • Virus Replication / genetics*

Substances

  • MicroRNAs
  • Mirn106 microRNA, mouse
  • Mirn150 microRNA, mouse
  • RNA, Small Interfering
  • Drosha protein, mouse
  • Ribonuclease III