In vitro and in vivo degradation of poly(D, L-lactide-co-glycolide)/amorphous calcium phosphate copolymer coated on metal stents

J Biomed Mater Res A. 2011 Mar 15;96(4):632-8. doi: 10.1002/jbm.a.33016. Epub 2011 Jan 25.

Abstract

The purpose of this study was to optimize a novel biodegradable polymer for drug eluting stent (DES) applications. Degradation profiles of different poly(D,L-lactide-co-glycolide)/amorphous calcium phosphate (PLGA/ACP) composites coated on stents were studied both in vitro and in vivo for three months. For the in vitro study, stents were immersed into the phosphate buffered saline (37 °C, pH 7.4) with constant shaking. The polymer weight loss was measured weekly and morphological changes were analyzed. The results demonstrated that approximately 60% of polymer was degraded within the three-month period and there was no significant difference between the different PLGA/ACP composites. However, the composite of 50% PLGA (65/35) with 50% ACP showed a slightly faster degradation rate than other composites. Morphologically, all stent surfaces changed from a micro-porous before degradation to a corrugated solid micro-net-like structure at two months post degradation. Based on in vitro results, 65% PLGA (65/35) with 35% ACP) coated stents were selected and implanted into rat aortas (n = 12) for the in vivo study. Microscopic observation showed that no composite was found on any of the implanted stents at 12 weeks post implantation, which indicated the selected PLGA/ACP composite is desired for DES applications.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / metabolism*
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / metabolism*
  • Drug-Eluting Stents*
  • Lactic Acid / chemistry
  • Lactic Acid / metabolism*
  • Materials Testing
  • Metals / chemistry
  • Metals / metabolism
  • Polyglycolic Acid / chemistry
  • Polyglycolic Acid / metabolism*
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polymers / chemistry
  • Polymers / metabolism*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Biocompatible Materials
  • Calcium Phosphates
  • Metals
  • Polymers
  • amorphous calcium phosphate
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid