Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast

Neelam Dhiman; Leslie Hall; Sherri L Wohlfiel; Seanne P Buckwalter; Nancy L Wengenack

doi:10.1128/JCM.02381-10

Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast

J Clin Microbiol. 2011 Apr;49(4):1614-6. doi: 10.1128/JCM.02381-10. Epub 2011 Jan 26.

Authors

Neelam Dhiman¹, Leslie Hall, Sherri L Wohlfiel, Seanne P Buckwalter, Nancy L Wengenack

Affiliation

¹ Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905, USA.

Abstract

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry was compared to phenotypic testing for yeast identification. MALDI-TOF mass spectrometry yielded 96.3% and 84.5% accurate species level identifications (spectral scores, ≥ 1.8) for 138 common and 103 archived strains of yeast. MALDI-TOF mass spectrometry is accurate, rapid (5.1 min of hands-on time/identification), and cost-effective ($0.50/sample) for yeast identification in the clinical laboratory.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Clinical Laboratory Techniques / economics
Clinical Laboratory Techniques / methods*
Costs and Cost Analysis
Humans
Mycology / economics
Mycology / methods*
Mycoses / diagnosis*
Mycoses / microbiology
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / economics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Time Factors
Yeasts / chemistry
Yeasts / classification
Yeasts / isolation & purification*