Abstract
Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Animals
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / metabolism
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Cell Line, Tumor
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Centrioles / chemistry*
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Centrioles / metabolism
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Centrioles / ultrastructure
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Centrosome / metabolism
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Chlamydomonas reinhardtii / chemistry
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Chlamydomonas reinhardtii / metabolism
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Chromosomal Proteins, Non-Histone / chemistry*
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Chromosomal Proteins, Non-Histone / metabolism
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Crystallography, X-Ray
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Flagella / metabolism
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Flagella / ultrastructure
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Humans
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Hydrophobic and Hydrophilic Interactions
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Models, Molecular
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Mutant Proteins / chemistry
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Point Mutation
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Protein Multimerization
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Protein Structure, Quaternary
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Protein Structure, Tertiary
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Zebrafish
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Zebrafish Proteins / chemistry*
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Zebrafish Proteins / metabolism
Substances
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Cell Cycle Proteins
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Chromosomal Proteins, Non-Histone
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Mutant Proteins
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Recombinant Proteins
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SASS6 protein, human
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Zebrafish Proteins
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sass6 protein, zebrafish