Evaluation of the immunogenicity of a transgenic tobacco plant expressing the recombinant fusion protein of GP5 of porcine reproductive and respiratory syndrome virus and B subunit of Escherichia coli heat-labile enterotoxin in pigs

Min-Yuan Chia; Shih-Hsuan Hsiao; Hui-Ting Chan; Yi-Yin Do; Pung-Ling Huang; Hui-Wen Chang; Yi-Chieh Tsai; Chun-Ming Lin; Victor Fei Pang; Chian-Ren Jeng

doi:10.1016/j.vetimm.2011.01.002

Evaluation of the immunogenicity of a transgenic tobacco plant expressing the recombinant fusion protein of GP5 of porcine reproductive and respiratory syndrome virus and B subunit of Escherichia coli heat-labile enterotoxin in pigs

Vet Immunol Immunopathol. 2011 Apr 15;140(3-4):215-25. doi: 10.1016/j.vetimm.2011.01.002. Epub 2011 Jan 11.

Authors

Min-Yuan Chia¹, Shih-Hsuan Hsiao, Hui-Ting Chan, Yi-Yin Do, Pung-Ling Huang, Hui-Wen Chang, Yi-Chieh Tsai, Chun-Ming Lin, Victor Fei Pang, Chian-Ren Jeng

Affiliation

¹ Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, Taipei 106, Taiwan, ROC.

PMID: 21277027
DOI: 10.1016/j.vetimm.2011.01.002

Abstract

Escherichia coli heat-labile enterotoxin B subunit (LTB) can be used as an adjuvant for co-administered antigens. Our previous study showed that the expression of neutralizing epitope GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) in transgenic tobacco plant (GP5-T) could induce PRRSV-specific immune responses in pigs. A transgenic tobacco plant co-expressing LTB and PRRSV GP5 as a fusion protein (LTB-GP5-T) was further constructed and its immunogenicity was evaluated. Pigs were given orally three consecutive doses of equal concentration of recombinant GP5 protein expressed in leaves of LTB-GP5-T or GP5-T at a 2-week interval and challenged with PRRSV at 7 weeks post-initial immunization. Pigs receiving LTB-GP5-T or GP5-T developed PRRSV-specific antibody- and cell-mediated immunity and showed significantly lower viremia and tissue viral load and milder lung lesions than wild type tobacco plant (W-T). The LTB-GP5-T-treated group had relatively higher immune responses than the GP5-T-treated group, although the differences were not statistically significant.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / administration & dosage
Administration, Oral
Animals
Antibodies, Viral / blood
Antibodies, Viral / metabolism
Bacterial Toxins / administration & dosage
Bacterial Toxins / genetics*
Bacterial Toxins / immunology*
Base Sequence
DNA Primers / genetics
Enterotoxins / administration & dosage
Enterotoxins / genetics*
Enterotoxins / immunology*
Escherichia coli / genetics
Escherichia coli / immunology
Escherichia coli Proteins / administration & dosage
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / immunology*
Lymphocyte Activation
Male
Nicotiana / genetics*
Nicotiana / immunology*
Plant Leaves / immunology
Plants, Genetically Modified
Porcine Reproductive and Respiratory Syndrome / immunology
Porcine Reproductive and Respiratory Syndrome / prevention & control
Porcine Reproductive and Respiratory Syndrome / virology
Porcine respiratory and reproductive syndrome virus / genetics*
Porcine respiratory and reproductive syndrome virus / immunology*
Porcine respiratory and reproductive syndrome virus / pathogenicity
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / immunology
Saliva / immunology
Sus scrofa
Swine
Vaccines, Edible / administration & dosage
Vaccines, Edible / genetics
Vaccines, Synthetic / administration & dosage
Vaccines, Synthetic / genetics
Viral Envelope Proteins / administration & dosage
Viral Envelope Proteins / genetics*
Viral Envelope Proteins / immunology*
Viral Load
Viral Vaccines / administration & dosage
Viral Vaccines / genetics

Substances

Adjuvants, Immunologic
Antibodies, Viral
Bacterial Toxins
DNA Primers
Enterotoxins
Escherichia coli Proteins
Recombinant Fusion Proteins
Vaccines, Edible
Vaccines, Synthetic
Viral Envelope Proteins
Viral Vaccines
glycoprotein 5, PRRSV
heat-labile enterotoxin, E coli