Abstract
Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle
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Cattle Diseases / microbiology
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Chromosomes, Bacterial
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DNA Transposable Elements / genetics*
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DNA, Bacterial
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Escherichia coli O157 / genetics*
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Escherichia coli O157 / metabolism*
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Genetic Fitness
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Genotype
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Mutagenesis, Insertional
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Plasmids
Substances
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DNA Transposable Elements
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DNA, Bacterial