Inactivation of the ELIP1 and ELIP2 genes affects Arabidopsis seed germination

New Phytol. 2011 Jun;190(4):896-905. doi: 10.1111/j.1469-8137.2010.03637.x. Epub 2011 Feb 7.

Abstract

Light regulates Arabidopsis seed germination through the phyB/PIL5 (PHYTOCHROME INTERACTING FACTOR 3-LIKE 5) transduction pathway, and we have previously shown that the Dof transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a component of this pathway. By means of microarray analysis of dag1 and wild type developing siliques, we identified the EARLY LIGHT-INDUCED PROTEIN1 and 2 (ELIP1 and ELIP2) genes among those deregulated in the loss-of-function dag1 mutant. We analysed seed germination of elip single and double mutants, of elip dag1 double mutants as well as of elip1 elip2 dag1 triple mutant under different environmental conditions. We show that ELIP1 and ELIP2 are involved in opposite ways in the control of this developmental process, in particular under abiotic (light, temperature, salt) stress conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / radiation effects
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant
  • Gene Silencing
  • Germination / radiation effects*
  • Light
  • Microarray Analysis
  • Mutation
  • Seeds / growth & development*
  • Seeds / metabolism
  • Seeds / radiation effects
  • Stress, Physiological
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Arabidopsis Proteins
  • DAG1 protein, Arabidopsis
  • DNA-Binding Proteins
  • ELIP protein, Arabidopsis
  • ELIP2 protein, Arabidopsis
  • Transcription Factors