Aim: To observe the killing effect of irradiated peripheral blood mononuclear cells (PBMCs) at low dose combined with (-)-gossypol on cultured human prostate cancer LNCaP cells.
Methods: Human PBMCs were irradiated by gamma ray at 1 gray, the irradiation dose rate was 17 Gy/min. The experiments were divided into control group (LNCaP cells only), LNCaP cells with irradiated and non-irradiated PBMCs co-culture groups, (-)-gossypol treatment group, irradiated PBMCs and (-)-gossypol co-treatment group. Acridine orange/ethidium bromide (AO/EB) staining and MTT method were used to observe the killing effect of PBMCs and/or (-)-gossypol.
Results: The killing activity of irradiated PBMCs group and (-)-gossypol treatment group were obviously increased and were higher than that of non-irradiated group (P < 0.05). The killing activity of combined group was much higher than that of irradiated group and ApoG2 treatment group (P < 0.01).
Conclusion: Irradiated PBMCs at low dose combined with (-)-gossypol can enhance the anti-tumor effects obviously.