Abstract
To study the clinical relevance of undifferentiated tumour cells in astrocytic gliomas we employed a large tumour tissue microarray (n=283) with corresponding clinical data and analyzed the expression of Nestin and Sox-2, which mark undifferentiated stem- and progenitor cells in the normal brain. Both markers were expressed abundantly and staining of nestin significantly increased with WHO grade. Further, nestin and Sox-2 immunoreactivity was significantly associated with tumour cell proliferation and nestin expression was independently associated with poor patient survival. Our findings suggest that immature glioma cells are involved in tumour growth and tumour progression and significantly impact on patient prognosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Astrocytoma / genetics
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Astrocytoma / metabolism*
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Astrocytoma / mortality
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Astrocytoma / pathology
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Biomarkers, Tumor / analysis*
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Brain / metabolism
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Brain / pathology
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Brain Neoplasms / genetics
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Brain Neoplasms / metabolism*
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Brain Neoplasms / mortality
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Brain Neoplasms / pathology
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Cell Proliferation
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Disease-Free Survival
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Female
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Gene Expression
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Humans
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Immunohistochemistry
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Intermediate Filament Proteins / genetics
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Intermediate Filament Proteins / metabolism*
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Longitudinal Studies
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Male
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Microarray Analysis
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Middle Aged
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Nestin
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Prognosis
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SOXB1 Transcription Factors / genetics
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SOXB1 Transcription Factors / metabolism*
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Stem Cells / metabolism
Substances
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Biomarkers, Tumor
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Intermediate Filament Proteins
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NES protein, human
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Nerve Tissue Proteins
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Nestin
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SOXB1 Transcription Factors