Abstract
Human bocavirus 2 (HBoV2) is a parvovirus that has been recently identified in stool samples from children. Any association between the virus and clinical disease is unclear. A rapid, reliable diagnostic method is necessary to address this issue. In this study, we developed a sensitive and specific HBoV2 quantitative real-time PCR assay that targets the HBoV2 NP-1 gene, based on the TaqMan method. The assay could reproducibly detect 10 copies of a recombinant DNA plasmid containing a partial region of the HBoV2 genome, with a dynamic range of 8 log units (10(1) to 10(8) copies). A clinical evaluation detected HBoV2 in 85 (24.6%) of 345 children with gastroenteritis, with viral loads ranging from 1.67 × 10(2) to 4.27 × 10(9) copies per ml of stool specimen.
Publication types
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Child, Preschool
-
Clinical Laboratory Techniques / methods*
-
DNA Primers / genetics
-
DNA, Viral / chemistry
-
DNA, Viral / genetics
-
Female
-
Human bocavirus / classification
-
Human bocavirus / genetics
-
Human bocavirus / isolation & purification*
-
Humans
-
Infant
-
Male
-
Molecular Sequence Data
-
Parvoviridae Infections / diagnosis*
-
Parvoviridae Infections / virology
-
Polymerase Chain Reaction / methods*
-
Sensitivity and Specificity
-
Sequence Analysis, DNA
-
Virology / methods*
Associated data
-
GENBANK/GU138990
-
GENBANK/GU138991
-
GENBANK/GU138992
-
GENBANK/HM853485
-
GENBANK/HM853486
-
GENBANK/HM853487
-
GENBANK/HM853488
-
GENBANK/HM853489
-
GENBANK/HM853490
-
GENBANK/HM853491
-
GENBANK/HM853492
-
GENBANK/HM853493
-
GENBANK/HM853494
-
GENBANK/HM853495
-
GENBANK/HM853496
-
GENBANK/HM853497
-
GENBANK/HM853498
-
GENBANK/HM853499
-
GENBANK/HM853500
-
GENBANK/HM853501
-
GENBANK/HM853502
-
GENBANK/HM853503
-
GENBANK/HM853504
-
GENBANK/HM853505
-
GENBANK/HM853506
-
GENBANK/HM853507
-
GENBANK/HM853508
-
GENBANK/HM853509
-
GENBANK/HM853510
-
GENBANK/HM853511
-
GENBANK/HM853512
-
GENBANK/HM853513
-
GENBANK/HM853514
-
GENBANK/HM853515
-
GENBANK/HM853516
-
GENBANK/HM853517
-
GENBANK/HM853518
-
GENBANK/HM853519
-
GENBANK/HM853520
-
GENBANK/HM853521
-
GENBANK/HM853522
-
GENBANK/HM853523
-
GENBANK/HM853524
-
GENBANK/HM853525
-
GENBANK/HQ012667
-
GENBANK/HQ012668
-
GENBANK/HQ012669
-
GENBANK/HQ012670
-
GENBANK/HQ012671
-
GENBANK/HQ012672
-
GENBANK/HQ012673
-
GENBANK/HQ012674
-
GENBANK/HQ012675
-
GENBANK/HQ012676
-
GENBANK/HQ012677
-
GENBANK/HQ141389
-
GENBANK/HQ141390
-
GENBANK/HQ141391
-
GENBANK/HQ141392
-
GENBANK/HQ141393
-
GENBANK/HQ141394
-
GENBANK/HQ141395
-
GENBANK/HQ398863
-
GENBANK/HQ398864
-
GENBANK/HQ398865
-
GENBANK/HQ398866
-
GENBANK/HQ398867
-
GENBANK/HQ398868
-
GENBANK/HQ398869
-
GENBANK/HQ398870