Abstract
Coronins are WD repeat-containing proteins highly conserved in the eukaryotic kingdom implicated in the regulation of F-actin. Mammalian coronin 1, one of the most conserved isoforms expressed in leukocytes, regulates survival of T cells, which has been suggested to be due to its role in preventing F-actin-induced apoptosis. In this study, we come to a different conclusion. We show that coronin 1 does not modulate F-actin and that induction of F-actin failed to induce apoptosis. Instead, coronin 1 was required for providing prosurvival signals, in the absence of which T cells rapidly underwent apoptosis. These results argue against a role for coronin 1 in F-actin-mediated T cell apoptosis and establish coronin 1 as an essential regulator of the balance between prosurvival and proapoptotic signals in naive T cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism*
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Actins / physiology
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Animals
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Apoptosis Regulatory Proteins / metabolism*
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Apoptosis Regulatory Proteins / physiology
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / immunology*
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CD4-Positive T-Lymphocytes / metabolism
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Calcineurin / metabolism
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Calcineurin / physiology
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Calcium Signaling / genetics
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Calcium Signaling / immunology*
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Cell Movement / genetics
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Cell Movement / immunology*
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Cell Survival / genetics
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Cell Survival / immunology*
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Homeostasis / genetics
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Homeostasis / immunology*
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Ligands
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Microfilament Proteins / deficiency
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Microfilament Proteins / genetics
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Microfilament Proteins / physiology*
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Receptors, Antigen, T-Cell / metabolism
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Resting Phase, Cell Cycle / genetics
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Resting Phase, Cell Cycle / immunology
Substances
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Actins
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Apoptosis Regulatory Proteins
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Ligands
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Microfilament Proteins
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Receptors, Antigen, T-Cell
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coronin proteins
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Calcineurin