An Ad5-vectored HIV-1 vaccine elicits cell-mediated immunity but does not affect disease progression in HIV-1-infected male subjects: results from a randomized placebo-controlled trial (the Step study)

J Infect Dis. 2011 Mar 15;203(6):765-72. doi: 10.1093/infdis/jiq114.

Abstract

Background: The Step study was a randomized trial to determine whether an adenovirus type 5 (Ad5) vector vaccine, which elicits T cell immunity, can lead to control of human immunodeficiency virus (HIV) replication in participants who became HIV-infected after vaccination.

Methods: We evaluated the effect of the vaccine on trends in HIV viral load, CD4+ T cell counts, time to initiation of antiretroviral therapy (ART), and AIDS-free survival in 87 male participants who became infected with HIV during the Step study and who had a median of 24 months of post-infection follow-up.

Results: There was no overall effect of vaccine on mean log(10) viral load (estimated difference between groups, -0.11; P = .47). In a subset of subjects with protective HLA types (B27, B57, B58), mean HIV-1 RNA level over time was lower among vaccine recipients. There was no significant difference in CD4+ T cell counts, time to ART initiation, or in AIDS-free survival between HIV-1-infected subjects who received vaccine versus those who received placebo.

Conclusions: HIV RNA levels, CD4+ T cell counts, time to initiation of ART, and AIDS-free survival were similar in vaccine and placebo recipients. There may have been a favorable effect of vaccine on HIV-1 RNA levels in participants with HLA types associated with better control of HIV-1.

Trial registration: ClinicalTrials.gov NCT00095576.

Publication types

  • Multicenter Study
  • Randomized Controlled Trial
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines / immunology*
  • Adenoviruses, Human / genetics
  • Adenoviruses, Human / immunology
  • Adult
  • Anti-Retroviral Agents / therapeutic use
  • Blotting, Western
  • CD4 Lymphocyte Count
  • CD4-Positive T-Lymphocytes / immunology
  • Circumcision, Male
  • Disease-Free Survival
  • Double-Blind Method
  • Genetic Vectors
  • HIV Infections / drug therapy
  • HIV Infections / immunology
  • HIV Infections / prevention & control*
  • HIV-1 / genetics
  • HIV-1 / immunology*
  • HIV-1 / physiology
  • Humans
  • Immunity, Cellular
  • Male
  • Middle Aged
  • Placebos
  • Proportional Hazards Models
  • RNA, Viral / blood
  • RNA, Viral / immunology
  • Viral Load / drug effects
  • Young Adult

Substances

  • AIDS Vaccines
  • Anti-Retroviral Agents
  • Placebos
  • RNA, Viral

Associated data

  • ClinicalTrials.gov/NCT00095576

Grants and funding