A multiplex polymerase chain reaction method for rapid pneumococcal serotype determination in childhood empyema

Naouale Maataoui; Philippe Bidet; Catherine Doit; Agathe De Lauzanne; Mathie Lorrot; Patricia Mariani-Kurkdjian; Albert Faye; Edouard Bingen

doi:10.1016/j.diagmicrobio.2010.10.001

A multiplex polymerase chain reaction method for rapid pneumococcal serotype determination in childhood empyema

Diagn Microbiol Infect Dis. 2011 Mar;69(3):245-9. doi: 10.1016/j.diagmicrobio.2010.10.001.

Authors

Naouale Maataoui¹, Philippe Bidet, Catherine Doit, Agathe De Lauzanne, Mathie Lorrot, Patricia Mariani-Kurkdjian, Albert Faye, Edouard Bingen

Affiliation

¹ Service de Microbiologie, Hôpital Robert-Debré (APHP), 75019 Paris, Université Denis Diderot-Paris 7, France.

PMID: 21353946
DOI: 10.1016/j.diagmicrobio.2010.10.001

Abstract

Nonvaccine serotypes are increasingly responsible for pneumococcal empyema. We developed a one-step polymerase chain reaction for identifying all serotypes covered by the 13-valent conjugate vaccine. Over 1 year, we identified serotypes 19A (n = 6), 1 (n = 2), 3 (n = 1) and 7F/A (n = 2), both in culture-positive and culture-negative pleural fluid from 11 children with empyema.

MeSH terms

Adolescent
Child
Child, Preschool
Empyema, Pleural / microbiology*
Female
Humans
Infant
Male
Pleural Effusion / microbiology
Pneumococcal Infections / microbiology*
Pneumococcal Vaccines
Polymerase Chain Reaction / methods*
Serotyping / methods*
Streptococcus pneumoniae / classification*

Substances

Pneumococcal Vaccines