Spontaneous reactive astrogliosis in the dentate gyrus of Bax-deficient mice

Mol Cells. 2011 Apr;31(4):379-83. doi: 10.1007/s10059-011-0319-9. Epub 2011 Feb 22.

Abstract

Astrocytes play critical roles in many aspects of brain functions via modulation of neurotransmission, metabolism, and structural remodeling in response to physiological or pathological stimuli. Activation of astrocytes is a common phenomenon in many brain pathologies such as stroke, trauma, and neurodegenerative diseases. In this study, we found that gene deletion of the pro-apoptotic gene Bax (Bax-knockout) resulted in a spontaneous reactive astrogliosis in the dentate gyrus, as evidenced by the increased number/volume of astrocytes and cytoplasmic localization of the Olig2 protein. On the other hand, there was no evidence for microglial activation in the dentate gyrus of Bax-knockout mice. Previously, we reported that Bax-knockout mice failed to execute programmed cell death of adult-produced neurons, but the surplus neurons eventually impaired normal synaptic connections and dendritic arborization of dentate gyrus neurons. Therefore, we propose that the reactive astrocytes in the Bax knockout mice may play a role in tissue remodeling of the dentate gyrus following a failure in the programmed cell death of adult-produced neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / cytology*
  • Basic Helix-Loop-Helix Transcription Factors / metabolism
  • Calcium-Binding Proteins / metabolism
  • Cell Proliferation*
  • Dentate Gyrus / cytology*
  • Dentate Gyrus / physiology
  • Gene Deletion
  • Glial Fibrillary Acidic Protein
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microfilament Proteins / metabolism
  • Microglia / metabolism
  • Nerve Tissue Proteins / metabolism
  • Oligodendrocyte Transcription Factor 2
  • bcl-2-Associated X Protein / genetics*

Substances

  • Aif1 protein, mouse
  • Basic Helix-Loop-Helix Transcription Factors
  • Bax protein, mouse
  • Calcium-Binding Proteins
  • Glial Fibrillary Acidic Protein
  • Microfilament Proteins
  • Nerve Tissue Proteins
  • Olig2 protein, mouse
  • Oligodendrocyte Transcription Factor 2
  • bcl-2-Associated X Protein
  • glial fibrillary astrocytic protein, mouse